Bioluminescence imaging to monitor the in vivo distribution of administered mesenchymal stem cells in acute kidney injury

摘要

Effective and targeted delivery of cells to injured organs is critical to the development of cell therapies. However, currently available in vivo cell tracking methods still lack sufficient sensitivity and specificity. We examined, therefore, whether a highly sensitive and specific bioluminescence method is suitable to noninvasively image the organ distribution of administered mesenchymal stem cells (MSCs) in vivo. MSCs were transfected with a luciferaseeomycin phosphotransferase construct (luceo-MSC).