A pear l-aminocyclopropane-i-carboxylate synthase gene preferentially expressed in leaves

摘要

1-Aminocyclopropane-l-carboxylate (ACC) synthase (ACS) catalyzes the conversion of S-adenosyl-L-methionine to ACC in the ethylene biosynthetic pathway. A cDNA clone encoding a putative ACS protein was isolated from a cDNA library produced using mRNA from pear {Pyrus pyrifolia). The cDNA clone, designated PpACS (GenBank accession No. JQ284383), comprised an open reading frame (ORF) of 1,341 bp encoding a protein of 446 amino acids that shares high similarity with the known plant ACSs. RT-PCR analysisindicated that the PpACS gene was preferentially expressed in pear leaves. The transcript of PpACS gene was accumulated at relatively high levels in anthers. Expression was detected in shoot at relatively low levels, but was not detected in fruit of pear. These results suggested that the PpACS may participate in the regulation of ethylene production in pear leaves and anthers.