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Impact of Reduced Rhodopsin Expression on the Structure of Rod Outer Segment Disc Membranes

机译:视紫红质表达减少对杆外节段膜结构的影响。

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Rhodopsin is the light receptor embedded in rod outer segment (ROS) disc membrane of Photoreceptor cells that initiates vision via phototransduction. The relationship between rhodopsin expression and the formation of menibrahe structures in the ROS is unclear but important to better understand both normal function and pathological conditions. To determine the impact of reduced rhodepsin expression on the structure of ROS discs and The supra, molecular Organization of rhodopSin, ROS disc membrane samples from heterozygous rhodopsin knockout mite were examined by atomic force microscopy. Similar to rhodopsin in wild-type mice, rhodopsin formed' nanodomains in ROS disc membranes of heterozygous knockout mice. The reduced rhodopsin expression in heterozygous knockout mice resulted in ROS disc membranes that Were smaller compared to those in wild-type mice at all ages tested. Changes in ROS disc membrane properties were observed between 4 and 6 weeks of age in heterozygous knockout mice that were not present in age-matched Wild-type mice. In 4 week old mite, the number and density of rhodopsin in ROS disc membranes was lower than that in agematched wild-type mice. In contrast, 6 and 8 week old mice had more thodopsin molecules present in disc membranes compared to 4 week old mice, which resulted in rhodopsin densities similar to those found in age matched wild-type mice. Thus, mechanisms appear to be present that maintain a constant density of rhodopsin Within ROS disc Membranes even when reducing the expression of the light receptor by about half. These adaptive mechanisms, however, only occur after 4 weeks of age.
机译:视紫红质是嵌入光感受器细胞的杆外节段(ROS)盘状膜中的光受体,可通过光转导引发视觉。视紫红质表达与ROS中脑膜结构的关系尚不清楚,但对于更好地了解正常功能和病理状况很重要。为了确定减少的视紫红质表达对ROS盘的结构的影响,通过原子力显微镜检查来自杂合的视紫红质基因敲除螨的视紫红质的分子组织,上述ROS盘膜样品。与野生型小鼠中的视紫红质相似,视紫红质在杂合敲除小鼠的ROS盘膜中形成了纳米结构域。在所有测试年龄的杂合敲除小鼠中视紫红质表达的降低导致ROS盘膜比野生型小鼠的膜小。在年龄匹配的野生型小鼠中不存在的杂合敲除小鼠中,在4至6周龄之间观察到ROS盘膜性质的变化。在4周龄的螨中,ROS圆盘膜中视紫红质的数量和密度低于年龄匹配的野生型小鼠。相比之下,与4周龄小鼠相比,6周龄和8周龄小鼠的视盘膜中存在更多的视紫红质分子,这导致视紫红质的密度与年龄匹配的野生型小鼠相似。因此,似乎存在即使在将光受体的表达减少约一半的情况下仍保持ROS盘膜内视紫红质的恒定密度的机制。但是,这些适应性机制仅在4周龄后出现。

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