Transient Kinetic Analysis of L-Serine Interaction with Escherichia coli D-3-Phosphoglycerate Dehydrogenase Containing Amino Acid Mutations in the Hinge Regions

摘要

In Escherichia coil D-3-phosphoglycerate dehydrogenase, the amino acid sequences G294-G295 and G336-G337. are found between structural domains and appear to function as hinge regions. Mutagenesis studies of these sequences showed that bulky side chains had significant effects on the kinetic properties of the enzyme. Placement of a tryptophanyl residue near the serine binding site (W139F/E360W) allows serine binding to be monitored by fluorescence quenching analysis. Pre-steady-state analysis has demonstrated that serine binds to two forms of the free enzyme, E and E+. " Conversion of Gly-336 to valine has its main effect on the K-d of serine binding to one form of the free enzyme (E) while maintaining the cooperativity of t binding observed in the native enzyme. Conversion of Gly-294 to valine eliminates a rate limiting conformational change that follows serine binding to E. The conformational change between the two forms of free enzyme is maintained, but the Hill coefficient for cooperativity is significantly lowered. The data indicate that the cooperative transmission induced by serine binding is transmitted through the Gly294-Gly295 hinge region to the opposite serine binding interface and that this is most likely propagated by way of the " substrate binding domain-regulatory domain interface. In the G294 mutant enzyme, both serine bound species, E.Ser and E*.Ser are present in significant amounts indicating that cooperativity of serine binding does not result from the binding to two different forms. The data also suggest that the E* form may be inactive even when serine is not bound.