A method for preserving monoclonal marker expression in bone marrow biopsies from haematological disorders using a glycol methacrylate cold-embedding technique

摘要

We report a simple glycol methacrylate (GMA) cold embedding technique that can be used on bone marrow biopsy core. The biopsies were fixed in cold Bouin's solution, dehydrated in cold methanol followed by infiltration and embedding in cold GMA then poly merised at 4deg under vacuum. This provided an effective cooling system, which preserved a wide range of the cell antigens by preventing heat damage due to excessive rise in temperature. This might be a useful diagnostic tool for the analyses of bone morphology and immuno-his-tochemistry on the same section. We have described a method of bone marrow biopsy which allows us to achieve excellent antigenic preservation and high-quality observation of morphological details in relation to expression with immuno-histochemical markers.