Magnetic-Bead-Based Microfluidic Systems for Fast Detection of Infectious Disease: Dengue Virus as an Example

摘要

The current study reports an innovative miniature microfluidic system for rapid detection of infectious diseases. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses, the target virus can be recognized and further purified by a magnetic field generated by the built-in microcoils. The purified target virus can be then lysed to release ribonucleic acid (RNA) for the subsequent nucleic acid amplification process such as reverse transcription polymerase chain reaction (RT-PCR) processes. Experimental results showed that the dengue viruses (serotype 1-4) can be successfully purified and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of viral RNA can also be completed utilizing the on-chip temperature control module in an automatic manner with the sensitivity as high as 100 PFU/mL. As a whole, the developed system may provide a potential micro-total-analysis-system (μ-TAS) for bio-sample pre-treatment and the fast diagnosis of infectious diseases.