Polyphenol extraction from eight Perilla frutescens cultivars

摘要

Eight cultivars of Perilla frutescens (L.) (var. frutescens and var. crispa) Britt. belonging to red and green varieties were harvested in China and Japan. Water extractions of dried plants were investigated at a small industrial scale, and their polyphenols contents were determined, identified and compared. Three cinnamic derivatives (coumaroyl tartaric acid, caffeic acid and rosmar-inic acid) ranged from 0.1 to 11 mg/g dry weight. Six fiavonoids (apigenin 7-O-caffeoylglucoside, scutellarein 7-O-diglucuronide, luteolin 7-O-diglucuronide, apigenin 7-O-diglucuro'nide, luteolin 7-O-glucuronide, and scutellarein 7-O-glucuronide) ranged from 3.5 to 18.5 mg/g. Six anthocyanins (0.7-2 mg/g) were detected in only red-green or red cultivars, including cw-shisonin, shisonin, malonylshisonin and cyanidin 3-O-(E)-caffeoylglucoside-5-O-malonylglucoside. The eight cultivars were classified into four chemotypes: two rich in phenolic acids (20-43%) and flavones (50-80%), with or without anthocyanins (0-7%) and two with mainly flavones (90-98%), with or without anthocyanins (0-7%). These extracts could be used for colored water beverages or various antioxidant pharmaceutical/biomedical applications.