Experimentally induced psoriatic lesion associates with interleukin (IL)-6 in mast cells and appearance of dermal cells expressing IL-33 and IL-6 receptor

摘要

Mast cells are involved in the development of psoriatic lesion, but it is not known how mast cells are activated or whether mast cell cytokines are expressed during the lesion development. In this study, the K?bner reaction was induced in uninvolved psoriatic skin of 18 patients using the tape-stripping technique, and a sequence of biopsies was collected at 0 days, 2h and 3 days or at 0 days, 1 day and 7 days for histochemical analysis. Eight patients developed the K?bner reaction verified at the follow-up visit 2-2·5 weeks later. No significant differences were observed in total tryptase + mast cells, psoriasis area and severity index and age/sex. Instead, the percentage of tryptase + mast cells showing interleukin (IL)-6 immunoreactivity was significantly higher in biopsies from K?bner-positive patients than in those from K?bner-negative patients. IL-33 is a known inducer of IL-6 in mast cells, and the number of IL-33 + cells increased significantly in K?bner-positive dermal skin at days 3-7. The number of dermal cells with IL-6 receptor (IL-6R, CD126) also increased in K?bner-positive skin at days 3-7. Unexpectedly, the number of IL-6R + cells was even higher in K?bner-negative skin at days 3-7. In the chronic plaque of 10 other psoriatic patients, the numbers of IL-6 + mast cells and dermal cells showing IL-6R were higher than those in the non-lesional skin. In conclusion, the positive K?bner reaction is associated with IL-6 in mast cells and appearance of IL-6R + and IL-33 + dermal cells. This suggests that a previously unrecognized vicious circle may develop in the early psoriatic lesion.