EFFECTS OF DIETARY DUNALIELLA SALINA EXTRACT AND HIGHLY UNSATURATED FATTY ACIDS ON THE FECUNDITY AND LIPID CONTENT OF POND-REARED PENAEUS JAPONICUS BROOD-STOCK

摘要

Five basic diets containing fresh squid meat and trash fish were supplemented with different amount of Dunaleilla salina extract (DSE) and highly unsaturated fatty acids (HUFA). Supplemented diets were fed to pond-reared Penaeus ja-ponicus broodstock.Diet A was solely squid and trash fish. Diets Bl and B2 were supplemented with 400 and 600mg DSE.kg"1 diet, respectively. Diets CI and C2 were supplemented with HUFA 5 and lOg.kg"1 and 400mg.kg~' DSE, respectively. The results showed that the group feddiet C2 had the best reproductive performance in all experimental groups. It had the highest proportion of spawns (73.5%) and egg production per female (589.0) than all the other experimental groups. The fatty acid composition strongly affected fecundity and stress tolerance of broodstock. The results showed that both HUFA and p-carotene DSE may play role in stress tolerance and reproductive performance