Latex agglutination test for detection of trypanosomosis in equines

摘要

A study was conducted to screen some horses in Haryana for trypanosomosis by monoclonal antibody based latex agglutination test (MAb-LAT). The latex reagent used in the present study contained suspension of latex microbeads, coated with saiti-Trypanosoma evansi murine monoclonal antibody. Seventy one horse blood samples were screened initially by wet blood film (WBF) and their corresponding serum samples were subjected to MAb-LAT. Some of these samples (n=16) were also examined by microhaematocrit centrifugation technique (MHCT). None of the blood samples showed the presence of T. evansi by WBF, whereas MHCT could detect 1 (6.25%) sample positive. However, 33 samples (46.48 %) were found positive by MAb-LAT. This study has revealed that MAb-LAT is more sensitive than the conventional parasitological examination for detection of trypanosomosis in horses. Moreover MAb-LAT is simple to perform, rapid, cost-effective and does not require sophisticated equipment for reading the results and hence can beused as a field-level test.