Comparison of thrombin generation for paired-platelet-rich plasma collected with and without corn trypsin inhibitor from hemophiliacs treated with factor-VIII inhibitor bypassing agents

摘要

Thrombin generation (TG) assays are increasingly used to investigate the coagulopathy associated with hemophilia and allied disorders and the hypercoagulability associated with clinical conditions at increased thrombosis risk. Although standardization is not yet achieved [1,2], these tests are usually carried out by triggering coagulation with small amounts of tissue factor (TF) with or without exogenous negatively charged phospholipids according to whether the test is performed in platelet-free or platelet-rich plasma. Concentrations of phospholipids/TF, blood drawing and plasma preparation are crucially related to the test results and may affect conclusions stemming from studies employing the test [3].