Enrichment of Hazelnut Oil with Long-Chain n-3 PUFA by Lipase-Catalyzed Acidolysis:Optimization by Response Surface Methodology

摘要

Response surface methodology (RSM) was used to determine optimal conditions for the lipase-catalyzed enrichment of hazelnut oil by incorporating n-3 PUFA from menhaden oil.A four-factor,five-level central composite design was used,and hazelnut oil containing n-3 PUFA was successfully produced.The effects of incubation time,temperature,substrate molar ratio,and water content on the incorporation ratio were investigated.From the evaluation of response surface graphs,the optimal conditions for incorporation of long-chain n-3 PUFA into hazelnut oil were identified as 45-60 deg C for temperature,30-40 h for reaction time,1:1-2:1 (mol hazelnut oil/mol menhaden oil concentrate) for substrate molar ratio,and 3-5% (w/w) for water content.Experiments conducted at optimized conditions predicted by the model equation obtained from RSM yielded structured lipids with 19.6% n-3 PUFA.This value agreed well with that predicted by the model.This structured lipid containing PUFA may be nutritionally more beneficial than unmodified hazelnut oil.