ENZYMATIC ESTERIFICATION OF OMEGA-3 FATTY ACID CONCENTRATES FROM SEAL BLUBBER OIL WITH GLYCEROL

摘要

Enzymatic synthesis of acyglycerols directly from glycerol and an omega-3 fatty acid concentrate, prepared from seal blubber oil, in organic solvents was studied. Seven lipases were used as biocatalysts for esterification. lipase LP-401-AS from Chromobacterium viscosum showed the highest activity for esterification. Effects of reaction parameters, namely, temperature, time course, type of solvent, water content, amount of glycerol, enzyme load and solvent volume, were followed with lipase LP-401-AS as the biocatalyst of choice. Optimal reaction conditions were established, and the maximal degree of acylglycerol synthesis reached was 94.3%. The concentrations of monoacylglycerols, diacylglycerols, and triacylglycerols were 13.8, 43.1, and 37.4%, respectively. Therefore, acylglycerols containing predominantly omega-3 fatty acid concentrates may be easily synthesized directly via their reaction with glycerol. [References: 12]