Determination of ergovaline in endophyted seeds by high performan ce thin layer chromatography 9hptlc)

摘要

High performance thin layer chromatography (HPTLC) is used tp rapidly determine the presence and amount of ergovalin in seeds of Graminaceae infested with _Neotyphodium Coeno- phialum. Quinine is used as an internal standard. The mycotoxin is solubilized under acid conditions and extracted with chloro- form under alkaline conditions. Samples are sprayed on silica gel plates and detected by fluorescence with A., ( excitation) set at 318 nm and A.2 (emission) > 400 nm. The method developed is specific for endophyte-infested seeds, linear from 3 to l2 I.lg/g and is both repeatable and repro- ducible. The limit of detection is 0.5 I.lg/g and the limit of deter- mination (quantification) is 3.0 I.lg/g. This technique was applied to several batches of seeds obtained from pastures that mayor may not be infested with the fungus. Results were compared with results obtained using a standard High Performance Liquid Chromatography (HPLC) method.