Concomitant hpic method for determination of lamotrigine,carbamazepine,and 10,11-carbamazepine epoxide in plasma using dual uv 240-220 nm wavelength detection

摘要

This paper presents a rapid,low cost,specific,high-performance liquid chromatographic(HPLC)method using an internal standard(pipamperone:PIP)for concomitant determination of lamotrigine(LMG),carbamazepine(CBZ),and its major metablite,10,11-carbamazepine epoxide(EPOCBZ).Drug deterction is performed using a dual wavelength detection (200-240 nm)to check the purity of chromatographic peaks. Mean retention times for LMG,EPOCBZ,PIP,CBZwere 3.73,4.34,5.41,and 7.93 minutes,respectively.Recovery rations averaged 88.1 percent +- 1.0 for LMG,101.1 percent +- 1.4 for CBZ,101.0 percent +- 1.6 forEPOCBZ,and 97.2 percent +- 2.5 for PIP,respectively (n=6).In the range investigated both the intra-day and the inter-day percent relative standard devation were less thn 6.5 percent (n=6).Absorbance rations of 240 versus 220 nm (AR 240/220nm)averaged 0.401 +- 0.0037 of LMG,0.227+-0.0046 for EPOCBZ,0.525 +- 0.0042 for CBZ,and 3.234 +- 0.115 for PIP,respectively(n=4).Albeit,no interference was detected with any of the drugs(or metabolites)tested,which might be associated with LMG or CBZ;determination of AR 240/220nm is of interest in laboratory routime while minimizing the risk of possible interferences without additional cost and waste of time.