DEVELOPMENT OF A HPLC METHOD FOR THE DETERMINATION OF 17-BETA-ESTRADIOL-3-PHOSPHATE IN PHARMACEUTICAL PREPARATIONS

摘要

A validated reversed-phase high-performance liquid chromatographic (HPLC) procedure for the analysis of 17 beta-estradiol-3-phosphate is reported. In the development of this assay, several factors were evaluated including buffer ionic strength, mobile phase pH, ion-pairing concentration, organic composition, and column type. The described method is rapid and coupled with standard HPLC procedures leads to a selective, accurate, and reproducible assay. The peak area versus 17 beta-estradiol-3-phosphate concentration is linear over the range of 0.1 - 100 mu g/mL, with a detection limit of 0.02 mu g/mL.