Investigation of the flavonoids and phenolic acids of sambuci flos by thin-layer chromatography

摘要

The efficiency of ten thin-layer chromatographic (TLC) systems for the separation of flavonoids and phenolic acids identified in a methanolic extract of Sambuci rios has been investigated by three mathematical techniques: calculation of the information content derived from Shannon's equation; determination of discriminating power (DP) and formation of clusters and dendrogram. The most suitable mobile phases for TLC of the compounds investigated are ethyl acetate-methanol-formic acid-water, 100 + 13.5 + 2.5 + 10 (v/v) and ethyl acetate-formic acid-water, 8 + 1 + 1 (v/v).