Evaluation and Selection of Optimal Solvent and Solvent Combinations in Thin-layer Chromatography of Flavonoids and of Phenolic Acids of Zizyphus jujuba Mill

摘要

Uporabljeni su odgovarajući matematički postupci za vrednovanje razvijača i kombinacija razvijača u tankoslojnoj kromatografiji. Klasifikacija razvijača je provedena metodama numeričke taksonomije uz dodatne kriterije: izračunavanje koeficijenta DP, i srednjeg vlastitog sadržaja informacije. Metode su primjenjene na eksperimentalnim podacima (R_F vrijednostima) za flavonoide i fenolne kiseline dokazane u me-tanolnom ekstraktu čičimaka - Zizjphus jujuba Mili. Najprikladniji kromatografski razvijači za odvajanje i buduću izolaciju flavonoida i fenolnih kiselina čičimaka su: etilacetat : mravlja kiselina : octena kiselina : voda, 100 : 11 : 11 : 27 i etilacetat : mravlja kiselina : voda, 8 : 1 : 1.%A series of simple mathematical techniques for the evaluation of solvents and solvent combinations in thin-layer chromatography have been investigated. The classification has been carried out using the numerical taxonomy techniques and information content derived from Shannon's equation. The methods have been applied to an R_F data set of flavonoids and phenolic acids identified in the methanolic extract of Zizyphus jujuba Mill. The most suitable chro-matographic systems for the separation and future isolation of flavonoids and phenolic acids from Zizyphus jujuba Mill, are : ethyl acetate : formic acid : acetic acid : water, 100 : 11 : 11 : 27, and ethyl acetate : formic acid : water, 8 : 1 : 1.