Simultaneous Determination of Methocarbamo! and Its Related Substance (Guaifenesin) In Two Ternary Mixtures with Ibuprofen and DIciofenac Potassium by HPTLC Spectrodensltometric Method

摘要

Accurate, sensitive, and precise high-performance thin-layer cfaro-matographic (HPTLC) method was developed and validated for the determination of Methocarbamol (ME) and its related substance (guaifenesin (GU)) in two ternary mixtures with ibuprofen (IB) and diclofenac potassium. The method depends on separation and quantification of the studied drugs on TLC silica gel 60 F_254 plates using ethyl acetate-acetone-triethylamine-formic acid (62:35:6:0.3, by volume) as the developing system followed by densitometric measurement of the bands at 222 am for the first mixture containing methocarbmol, IB, and GU and at 278 nm for the second mixture containing methocarbmol, diclofenac potassium, and GU. The proposed methods were successfully applied for the determination of ME, IB, and diclofenac potassium in the presence of ME-related substance (GU) either in bulk powder or in their pharmaceutical formulations.