SIMULTANEOUS DETERMINATION OF METHOCARBAMOL AND ITS RELATED SUBSTANCE (GUAIFENESIN) IN TWO TERNARY MIXTURES WITH IBUPROFEN AND DICLOFENAC POTASSIUM BY RP-HPLC METHOD

摘要

Accurate, sensitive, and precise RP-HPLC methods were developed and validated for determination of Methocarbamol and its related substance (Guaifenesin) in two ternary mixtures with Ibuprofen and Diclofenac potassium. The first mixture containing Methocarbamol, Ibuprofen, and Guaifenesin was separated using Isocratic elution on a Phenomenex C₁₈ column using 0.05 M KH₂PO₄ (pH = 7): acetonitrile methanol (90:25:15, by volume) as a mobile phase at a flow rate of 1.2 mL.min−1 and UV detection at 220 nm. The second mixture containing Methocarbamol, Diclofenac potassium, and Guaifenesin was separated using Isocratic elution on a Phenomenex C₁₈ column using 0.05 M KH₂PO₄ (pH = 7): acetonitrile (80:30, v/v) as a mobile phase at a flow rate of 1.5 mL.min−1 and UV detection at 278 nm. The proposed methods were successfully applied for determination of Methocarbamol, Ibuprofen, and Diclofenac potassium in presence of Methocarbamol related substance (Guaifenesin) either in bulk powder or in its pharmaceutical formulation.View full textDownload full textKeywordsdiclofenac potassium, guaifenesin, ibuprofen, methocarbamol, RP-HPLCRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2011.629393