Facile internalization of paclitaxel on titania nanoparticles in human lung carcinoma cells after adsorption of serum proteins

摘要

The enhanced damage of human carcinoma cells was achieved using paclitaxel (PTX)- assembled TiO _2 nanoparticles (NPs). The crystalline structures and light absorption properties of the TiO _2 NPs were examined by X-ray diffraction patterns and ultraviolet-visible absorption spectroscopic tools. The fabrication of PTX and serum proteins on TiO _2 NPs via self-assembly was checked by diffuse reflectance infrared Fourier transform spectroscopy and dynamic light scattering measurements. The PTX-coated TiO _2 NPs appeared to be well adsorbed by serum proteins. The PTX-coated TiO _2 NPs were found to be well internalized in cancer cells as indicated by transmission electron microscopy. The intracellular aggregation of PTX-coated TiO _2 NPs was assumed to occur after endocytosis leading to an entrapment of NPs in an endosomal or a lysosomal structure. Human lung carcinoma A549 cancer cells were chosen to examine their viability after the cellular uptake of the PTXcoated TiO _2 NPs. A 3-(4,5-dimethylthiazol- 2-yl)-2,5- diphenyltetrazolium bromide cell viability assay test indicated that the cell viability should be affected by the concentration of TiO _2 NPs. After applying PTXcoated TiO _2 NPs (50 ppm (μg/mL)) incubating for 24 h, we observed a 30 % further decrease of cell viability for PTX alone in the low concentration range of 0.1-10 nM.