A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

Adams LaTonzia L.; Salee Parichat; Dionne Kim; Carroll Karen; Parrish Nicole

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A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

机译：一种使用MALDI-ToF MS鉴定分枝杆菌的新型蛋白质提取方法

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摘要

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard. (C) 2015 Elsevier B.V. All rights reserved.

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《Journal of Microbiological Methods》 |2015年第null期|共3页
作者
Adams LaTonzia L.; Salee Parichat; Dionne Kim; Carroll Karen; Parrish Nicole;
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正文语种 eng
中图分类微生物研究与微生物实验;
关键词
Mycobacterium; Protein extraction; MALDI-ToF MS;

机译：分枝杆菌;蛋白质提取;MALDI-ToF MS;

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1. Rapid one-step protein extraction method for the identification of mycobacteria using MALDI-TOF MS [J] . Rotcheewaphan Suwatchareeporn, Lemon Jamie K., Desai Uma U., Diagnostic microbiology and infectious disease . 2019,第4期

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2. A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS [J] . Adams LaTonzia L., Salee Parichat, Dionne Kim, Journal of Microbiological Methods . 2015,第Null期

机译：一种使用MALDI-ToF MS鉴定分枝杆菌的新型蛋白质提取方法
3. A novel liquid media mycobacteria extraction method for MALDI-TOF MS identification using VITEK? MS [J] . Erik Moreno, Eric Miller, Elizabeth Miller, Journal of Microbiological Methods . 2018,第期

机译：一种新型液体培养物分枝杆菌的MARDI-TOF MS鉴定使用VITEK的提取方法？多发性硬化症
4. On-tissue protein identification by MALDI-TOF MS imaging and MS/MS [C] . Heysun Maeng, Jongsik Lee, Yangsun Kim American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

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5. LC-MS based bio-analytical methods for DMPK studies, toxicity screening and identification of DNA/protein adducts in the presence of bioreactors [D] . Bajrami, Besnik 2009

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6. Developing Two Rapid Protein Extraction Methods Using Focused-Ultrasonication and Zirconia-Silica Beads for Filamentous Fungi Identification by MALDI-TOF MS [O] . Ya-Ting Ning, Wen-Hang Yang, Wei Zhang, 2021

机译：使用聚焦 - 超声波和氧化锆 - 二氧化硅珠制筛选两种快速蛋白质提取方法用于MARDI-TOF MS的丝状真菌鉴定
7. An improved simple method for the identification of Mycobacteria by MALDI-TOF MS (Matrix-Assisted Laser Desorption- Ionization mass spectrometry) [O] . Adela Alcolea-Medina, M. T. Cabezas Fernandez, N. Montiel, 2019

机译：通过MALDI-TOF MS（基质辅助激光解吸离子质谱法鉴定分枝杆菌的改进简单方法

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

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