SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome

摘要

We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose. Display Omitted