Effect of jasmonic acid on the root growth and the eleutheroside accumulation in the adventitious root culture of Eleutherococcus senticosus

摘要

This study was carried out to investigate the dose-dependent effect of jasmonic acid on both the adventitious root growth and the accumulation of various eleutherosides in the Eleutherococcus senticosus bioreactor culture. The highest biomass production (4.6g DW/L) was observed in the absence of jasmonic acid and the root growth was significantly decreased by increasing the jasmonic acid concentration. However, jasmonic acid stimulated the production of both eleutheroside B, E and El. The highest level of eleutheroside B and E (476.3 mu g/g DW and 676.0 mu g/g DW) was obtained by 0.5 mg/L jasmonic acid treatment, while eleutheroside E, was accumulated at the highest level by 0.01 mg/L jasmonic acid treatment. The highest content of total eleutheroside was 2468.6 mu g/L when 0.01 mg/L jasmonic acid was applied. In addition, when the adventitious roots were cultured with 0.01 mg/L jasmonic acid, the highest levels of eleutheroside B, E and E, were observed at the 6th, 8th and 4th day of culture, respectively.