Effect of Methyl Jasmonate on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Culture of Eleutherococcus senticosus

摘要

This study was carried out to investigate the dose-dependent effect of methyl jasmonate on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus adventitious roots. Thehighest biomass production (5.4 g DW/L) was observed in the absence of methyl jasmonate and the root growth was significantly decreased by increasing the methyl jasmonate concentration. However, methyl jasmonate stimulated the production of both eleutheroside B, E and E_1. The highest level of eleutheroside B (359.9 mu g/g DW) was obtained at 40 mu M of methyl jasmonate, while eleutheroside E and E, was accumulated at the highest level by the addition of 10 mu M of methyl jasmonate. Total eleutherosidewas increased up to 3818.1 mu g per liter when 10 mu M of methyl jasmonate was applied. In addition, when the adventitious roots were cultured with 20 mu M of methyl jasmonate, the highest levels of eleutheroside B, E and E_1 were observed at the 12th,3th and 9th days of culture, respectively.