Effects of Jasmonic Acid on Root Growth and Eleutheroside Accumulation in Adventitious Root Culture of Eleutherococcus koreanum

摘要

The influence of different doses of jasmonic acid on both biomass of adventitious roots and accumulation of secondary metabolite in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth (2.9 g Dry Weight/L) of adventitious roots was observed in the absence of jasmonic acid. The biomass of adventitious roots decreased with the increase in jasmonic acid dosage. High level of jasmonic acid efficiently stimulated the production of both eleutheroside B and E. The highest eleutheroside B (49.0 mu g/g DW) was observed at 0.4 mg/L jasmonic acid. Jasmonic acid at 0.2 mg/L led to the maximum accumulation of eleutheroside E (64.7 mu g/g DW). Whereas eleutheroside E, was maximally 19.0 mu g/g DW accumulated in the absence of jasmonic acid, the total eleutheroside (259.9 mu g/L) was the highest when 0.01 mg/L jasmonic acid was treated. In this study, the level of eleutheroside B decreased whereas the maximum levels of eleutheroside E and eleutheroside E_1 were observed at 10th and 4thday after jasmonic acid treatment.