Effect of Salicylic Acid on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Cultureof Eleutherococcus senticosus

摘要

This study was carried out to investigate the dose-dependent effect of salicylic acid on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus. The highest biomass production (5.4 g DW/L) was observed in the absence of salicylic acid, while the root growth was significantly decreased by increasing the concentration of salicylic acid. Salicylic acid stimulated the production of both eleutheroside B, E and E_1. The highest levels of eleutheroside B (179.5 ug/g DW), E (1169.9 mu g/g DW) and E_1 (45.4 mu g/g DW) were obtained by the addition of 80 uM of salicylic acid. The maximum eleutheroside production was 4975.8 mu g/L when salicylic acid was not added. In addition, when the adventitious roots were cultured in the basal medium supplemented with 80 uM of salicylic acid, the highest levels of eleutheroside B was observed at the 9th day, while eleutheroside E and E_1 were observed at the 6th day, respectively.