Genetic Data of Six STR Loci of Han Population in Henan Province (Central China)

摘要

Blood samples were collected from unrelated individuals of Chinese Han ethnic group in Zhengzhou of Henan. DNA was extracted using Chelex method (1). Target DNA of 0.5-2 ng was amplified using GeneAmp PCR System 9700 (Applied Biosystems,Foster City, CA) following the protocols described in the AmpFLSTR Identifiler~(TM) PCR amplification kits (PE Applied Biosystems) with the exception that the PCR volume was 25 mul. DNA typing was carried out with the ABI 3100 Genetic Analyzer (Applied Biosystems) within which PCR products were separated, and GeneScan analysis software (version 3.1.2) was used to analyze the results. Genotypes were determined by comparison with allelic ladder using Genotyper DNA fragment analysis software (version 2.5) (Applied Biosystems). Alleles were designated following the DNA recommendations of the DNA commission of the ISFH (2). Statistical parameters like power of discrimination, polymorphic information content, power of exclusion, paternity index, matching probability, and heterozygosity observed were calculated using PowerStats V12 (3-5). Test for Hardy-Weinberg equilibrium was carried out according to Hou's method (6). No deviation from Hardy-Weinberg equilibrium was observed.