METHOD OF DETECTING CANDIDATE GENES FOR POPULATION RESEARCH OF GENETIC POLYMORPHISM IN CHILDREN DWELLING IN STRONTIUM GEOCHEMICAL PROVINCE ENVIRONMENT

摘要

FIELD: medicine.;SUBSTANCE: invention relates to biochemistry and medicine, namely to method of detecting candidate genes for population research of genetic polymorphism in children, dwelling in strontium geochemical province environment. To this end blood is sampled in children, living in strontium geochemical province no less than 3 years. From this sample DNA is recovered and library of short pieces of DNA is created. Their hybridization with set of preset primers is made, which represent liquid DNA-biochip. Then hybridized sections are subjected to sequencing, stating actual sequence of nucleotides, composing genes and comparing sequence with reference sequence of nucleotides in genes. Deviations are determined in sequence, taking such deviations as associated with possible child health disorders under action of strontium. As above genes are used: CYP1A2, TLR4, TERT, FAS, FOXP3, TP53, MTHFR, SULT1A1, VEGF, ZMPSTE, SOD, SIRT3, NOS3, PPARD and CPOX. In sequence of nucleotides of each of said genes number of single-nucleotide polymorphisms is identified, and presence of such polymorphisms in gene in amount of 6 and more shows connection of such changed gene with strontium exposure acting on child in conditions of strontium geochemical province environment. This gene is accepted as candidate gene for further study of population genetic polymorphism in children, dwelling in strontium geochemical province environment.;EFFECT: present invention enables detection of candidate genes through genetic polymorphism in children, associated with effect of strontium, and further use of obtained information for population analysis for establishing at early stage of certain diseases, caused by disrupted gene.;1 cl, 3 tbl, 1 ex