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Antifungal effect of cinnamon essential oil on Byssochlamys fulva in liquid medium and tomato sauce

机译:肉桂精油对液体培养基和番茄酱中Byssochlamys fulva的抑菌作用

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This study was conducted to evaluate the antifungal activity of Cinnamomum cassia essential oils against Byssochalmys fulva in culture media and tomato sauce. The procedure had three steps; in the first step macrodilution method was used to determinethe minimum inhibitory concentration (MIC) of cinnamon essential oils against the heat resistance of mold in culture media. The results showed that cinnamon was a promising antifungal substance with MIC of 400 ppm. In the second step, sensory evaluationof tomato sauce treated with 150, 250, 350 ppm concentrations of essential oil was assessed for flavor and odor of the food samples. Sample with 150 ppm cinnamon oil was accepted by panelists. In the third step, B. fulva was inoculated in tomato sauce 3× 10-5 CFU/g and then 0, 150 ppm and the MIC concentration of essential oils were added to each sample and kept at 30 ± 0.5 °C for 2 months. In addition to these sample tests, sodium benzoate stopped growth of B. fulva at concentration of 250 ppm in culture media. Sodium benzoate at concentration of 250 ppm in tomato sauce was used as a positive control. Results showed that in the MIC concentrations of cinnamon essential oil and sodium benzoate fungal growth was completely retarded, but fungal growthwas inhibited up to 72 % at 150 ppm concentration of cinnamon essential oils after 2 months. The main components of cinnamon essential oils, identified by gas chromatography-mass spectrometry, were (E)-cinnamaldehyde, α-Copaene and delta-Cadinene.
机译:本研究旨在评估肉桂肉桂精油在培养基和番茄酱中对Byssochalmys fulva的抗真菌活性。该过程分为三个步骤:第一步,采用宏观稀释法确定肉桂精油对培养基中霉菌耐热性的最小抑制浓度(MIC)。结果表明,肉桂是一种很有前途的抗真菌物质,MIC为400 ppm。在第二步中,评估了用150、250、350 ppm浓度的精油处理过的番茄酱的感官评估,以评估食品样品的风味和气味。小组成员接受了含150 ppm肉桂油的样品。第三步,在番茄酱中接种B. fulva 3×10-5 CFU / g,然后将0,150 ppm和MIC精油浓度添加到每个样品中,并在30±0.5°C下保持2个月。 。除这些样品测试外,苯甲酸钠在培养基中以250 ppm的浓度阻止了富叶双歧杆菌的生长。番茄酱中浓度为250 ppm的苯甲酸钠用作阳性对照。结果表明,在MIC浓度下,肉桂精油和苯甲酸钠的真菌生长被完全抑制,但是在2个月后浓度为150 ppm的肉桂精油下,真菌的生长被抑制了高达72%。通过气相色谱-质谱法鉴定的肉桂精油的主要成分是(E)-肉桂醛,α-Copaene和δ-Cadinene。

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