Use of the direct epifluorescent filter technique for the enumeration of viable and total acetic acid bacteria from vinegar fermentation

摘要

A rapid epifluorescence staining method using the LIVE/DEAD(R) BacLight(TM) Bacterial Viability kit was applied to differentiate both viable and total counts of acetic acid bacteria in vinegar fermentation. The results obtained were compared with those from other measurement techniques: 4', 6-diamidine-2phenyl indole (DAPI) and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and colony counts. BacLight total counts were comparable to DAPI ( differing by <3.5%). BacLight viable counts were similar to CTC counts but considerable higher than colony-forming cells in plates. [References: 17]