High-performance liquid chromatography with nuclear magnetic resonance detection - A method for quantification of alpha- and gamma-linolenic acids in their mixtures with free fatty acids

摘要

While many naturally occurring mixtures of free fatty acids are conveniently analyzed by hyphenated technique of LC-NMR, a complete separation of alpha- and gamma-linolenic acids for their quantitative determination appears impossible at least by the methods of reversed phase HPLC. However, they can be differentiated and quantified from H-1 NMR spectra measured in the course of isocratic acetonitrile-chloroform (90: 10, with C-8 and C-18 columns in series) LC-NMR analysis without the Deed for any derivatization. (c) 2006 Elsevier B.V. All rights reserved.