Solvent and solid-phase extraction of natural and synthetic anabolic steroids in human urine

摘要

Liquid-liquid(using dichloromethane) and liquid-solid extraction processes (using disposable C_(18) cartridges) were applied to human urine samples spiked with 15 androgenic anabolic steroids (natural and synthetic).The extraction recoveries were assessed from different HPLC separations of anabolic steroids using water-acetonitrile mobile phase,andusing calibration graphs obtainedby injection into HPLC of standard samples of these compounds before and after extraction.The procedures,including sampelpreconcentration,showed wctraction efficiencies over 90% which were independent on a wide range of concentrations tested.Solid phase extraction yielded poor results for oximetolone,danazol and dehydroepiandrosteroe.For real urine samples,hydrolysis using #beta#-glucuronidase and washig using sodium hydroxide before and after solvent extraction,respectively,is recommended.