Hypothermia-induced platelet aggregation in human blood in an in vitro model: The dominant role of blood-material interactions

摘要

Hypothermia-induced platelet aggregation (HIPA) with or without neutrophil involvement may cause neurologic dysfunction during hypothermic surgery. We report the use of a previously developed model to study the contributions of several surfaces, surface area, shear rate, and blood-material exposure time to HIPA. Heparinized (1.5 u/mL) human blood was quenched to 24 deg C and passed (0.5 mL/min) through a 75-cm long 1/32" ID tubing of poly-vinylchloride (PVC), polyethylene (PE), polyurethane (PU), Teflon-FEP, or heparin (Duraflo)-coated PVC. The number of aggregates was measured by a light-scattering method, and the concentration of occlusive aggregates was assessed using constant-pressure filtration (50 mmHg). No differences were seen among PVC, PE, PU, or Teflon-FEP. The heparin-coated PVC tubing produced fewer occlusive aggregates, and heparin leaching from the coating was not the cause of the decrease in occlusive aggregates. Increasing surface area increased the number of aggregates, and increasing shear rates decreased the occlusiveness of those aggregates.