薤白皂苷对血小板聚集及血小板-中性粒细胞间相互作用的影响

摘要

Objective To investigate the effects of saponins from Allium Macrostemon Bunge Bulbs (SMBB) on platelet aggregation and platelet-neutrophil-interactions. Methods The effects of SMBB on platelet aggregation in SD rats were observed in vivo and in vitro. The adhesion of platelets to neutrophils was measured by rosette test. The effect of SMBB on activated platelet calcium levels was detected. Results Platelet aggregation induced by platelet activating factor (PAF), adenosine diphosphate (ADP) and arachidonic acid (AA) was significantly inhibited by SMBB in vitro concentration-dependent. Platelet aggregation induced by PAF, AA and ADP was significantly inhibited by 5 mg/kg of SMBB. SMBB could reduce the intracellular calcium concentration in the wash platelets. SMBB significantly reduced the adhesion between neutrophils and thrombin-activated platelets and inhibited neutrophil supernatant-induced platelet aggregation, with IC50of 2.7 μmol/L and 9.6 μmol/L, respectively. Conclusion SMBB can inhibit platelet aggregation in vitro and ex vivo, and inhibit the interactions between platelets and neutrophils.%目的 探讨薤白皂苷对血小板聚集及血小板-中性粒细胞间相互作用的影响.方法 分别于在体和离体环境下观察SD大鼠血小板聚集功能,采用玫瑰花环试验测定血小板与中性粒细胞的黏附能力,检测细胞溶质钙离子的浓度.结果 薤白皂苷离体环境下呈浓度依赖性抑制花生四烯酸(AA)、腺苷二磷酸(ADP)或血小板活化因子(PAF)诱导的血小板聚集;薤白皂苷在体环境下呈浓度依赖性抑制PAF、ADP及AA诱导的血小板聚集,5 mg/kg薤白皂苷明显抑制PAF、AA和ADP诱导的血小板聚集;薤白皂苷可降低洗涤血小板内钙离子浓度;薤白皂苷可降低中性粒细胞与凝血酶激活的血小板间的黏附,并抑制中性粒细胞上清液诱导的血小板聚集,其50%抑制浓度分别为2.7、9.6 μmol/L.结论 薤白皂苷体外和体内均可抑制血小板聚焦,抑制血小板-中性粒细胞间的相互作用.