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Comparative Studies of beta-carotene and Protein Production From Dunaliella salina Isolated From Lake Hoze-soltan, Iran

机译:伊朗胡泽索尔坦湖分离的杜氏盐藻中β-胡萝卜素和蛋白质生产的比较研究

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摘要

A novel Dunaliella salina (D. salina) was isolated from Hoze-soltan Lake, Iran. The investigated strain was capable of producing carotenoids and protein, using different NaCl concentrations and pH variations in Hoze-soltan saline water. The experiment was carried out within 42 days. The results indicated that protein production and pigmentation correlated well with growth rate. The optimum amount for growth, carotenogenesis, and protein production was determined in a culture with 10% NaCl and pH 8.5. After 42-days incubation, 14.95 +/- 0.6 mu g/mL of carotenoids and 186 +/- 8.6 mu g/mL of protein were obtained in this medium. Further observation revealed that growth was inhibited in all samples with 30% NaCl. The amount of each biosynthesized component by Dunaliella salina was in accordance with growth stage, cell number, and growth pattern of microalgae.
机译:从伊朗的Hoze-soltan湖中分离出一种新型杜氏盐藻(D. salina)。使用不同的NaCl浓度和Hoze-soltan盐水中的pH值变化,所研究的菌株能够产生类胡萝卜素和蛋白质。实验在42天内进行。结果表明,蛋白质的产生和色素沉着与生长速度密切相关。在含有10%NaCl和pH 8.5的培养物中确定了最佳的生长,类胡萝卜素生成和蛋白质产生量。温育42天后,在该培养基中获得14.95 +/-0.6μg/ mL的类胡萝卜素和186 +/-8.6μg/ mL的蛋白质。进一步观察发现,在所有样品中,使用30%NaCl抑制生长。杜氏盐藻(Dunaliella salina)的每种生物合成成分的数量均与微藻的生长阶段,细胞数量和生长方式一致。

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