Effect of P- Mercaptoethanol Supplement during In Vitro Maturation on IVM, IVF and Glutathione Level in Porcine Oocytes

摘要

Experiments were conducted to determine the effects of beta-mercaptoethanol (0-ME) supplements to the in vitro maturation (IVM) medium on in vitro fertilization (IVF) and intracellular glutathione (GSH) concentration. Porcine cumulus-intact oocytes were matured in TCM-199 medium containing porcine follicular fluid, sodium pyruvate, D-glucose, FBS, hormonal supplements, and 0-ME (0, 25, 50 and 100uM) for 36 to 46h. After culture, cumulus-free matured oocytes were co-incubated with epididymal spermatozoa for 18h. There were no significant differences in the maturation rate among treatment groups. However, increases (P<0.05) in intracellular GSH concentration before and after fertilization were observed in 50 uM 0-ME supplements to the IVM medium. Also, increases (P<0.05) in male pronuclear formation after IVF were observed in same treatment group. In conclusion, supplementing 0-ME into the IVM medium increased intracellular GSH concentrations and increased fertilization in vitro.