首页> 外文期刊>Biochimica et Biophysica Acta. Molecular and cell biology of Lipids >Contribution of different pathways to the supply of phosphatidylethanolamine and phosphatidylcholine to mitochondrial membranes of the yeast Saccharomyces cerevisiae
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Contribution of different pathways to the supply of phosphatidylethanolamine and phosphatidylcholine to mitochondrial membranes of the yeast Saccharomyces cerevisiae

机译:不同途径对酿酒酵母酵母线粒体膜的磷脂酰乙醇胺和磷脂酰胆碱供应的贡献

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In the yeast, three biosynthetic pathways lead to the formation of phosphatidylethanolamine (PtdEtn): (i) decarboxylation of phosphatidylserine (PtdSer) by phosphatidylserine decarboxylase 1 (Psd1p) in mitochondria; (ii) decarboxylation of PtdSer by Psd2p in a Golgi/vacuolar compartment; and (iii) the CDP-ethanolamine (CDP-Etn) branch of the Kennedy pathway. The major phospholipid of the yeast, phosphatidylcholine (PtdCho), is formed either by methylation of PtdEtn or via the CDP-choline branch of the Kennedy pathway. To study the contribution of these pathways to the supply of PtdEtn and PtdCho to mitochondrial membranes, labeling experiments in vivo with [H-3]serine and [C-14]ethanolamine, or with [H-3]serine and [C-14] choline, respectively, and subsequent cell fractionation were performed with psd1Delta and psd2Delta mutants. As shown by comparison of the labeling patterns of the different strains, the major source of cellular and mitochondrial PtdEtn is Psd1p. PtdEm formed by Psd2p or the CDP-Etn pathway, however, can be imported into mitochondria, although with moderate efficiency. In contrast to mitochondria, microsomal PtdEtn is mainly derived from the CDP-Etn pathway. PtdEm formed by Psd2p is the preferred substrate for PtdCho synthesis. PtdCho derived from the different pathways appears to be supplied to subcellular membranes from a single PtdCho pool. Thus, the different pathways of PtdEtn biosynthesis play different roles in the assembly of PtdEtn into cellular membranes. (C) 2004 Elsevier B.V All rights reserved.
机译:在酵母中,三种生物合成途径导致磷脂酰乙醇胺(PtdEtn)的形成:(i)线粒体中的磷脂酰丝氨酸脱羧酶1(Psd1p)使磷脂酰丝氨酸(PtdSer)脱羧; (ii)在高尔基体/真空室中Psd2p使PtdSer脱羧; (iii)肯尼迪途径的CDP-乙醇胺(CDP-Etn)分支。酵母的主要磷脂磷脂酰胆碱(PtdCho)是通过PtdEtn甲基化或通过肯尼迪途径的CDP-胆碱分支形成的。要研究这些途径对线粒体膜PtdEtn和PtdCho的供应的贡献,使用[H-3]丝氨酸和[C-14]乙醇胺或[H-3]丝氨酸和[C-14]进行体内标记实验分别用]胆碱和psd1Delta和psd2Delta突变体进行后续细胞分离。如通过比较不同菌株的标记模式所示,细胞和线粒体PtdEtn的主要来源是Psd1p。尽管效率中等,但由Psd2p或CDP-Etn途径形成的PtdEm可以导入线粒体。与线粒体相反,微粒体PtdEtn主要来自CDP-Etn途径。由Psd2p形成的PtdEm是PtdCho合成的优选底物。来自不同途径的PtdCho似乎是从单个PtdCho库中提供给亚细胞膜的。因此,PtdEtn生物合成的不同途径在PtdEtn组装到细胞膜中起着不同的作用。 (C)2004 Elsevier B.V保留所有权利。

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