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Biosynthesis of exopolysaccharides (EPS) on Pseudomonas Agar P Medium with sugarbeet molasses by Pseudomonas aeruginosa G1 and Pseudomonas putida G12 strains

机译:铜绿假单胞菌G1和恶臭假单胞菌G12菌株在带有甜菜糖蜜的假单胞菌琼脂P培养基上生物合成胞外多糖(EPS)

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摘要

In this study, the production of exopolysaccharides (EPS) by Pseudomonas aeruginosa G1 and Pseudomonas putida G12 strains on Pseudomonas Agar P Medium with 2% glycerol was investigated. EPS yields from these strains were detected (192 mg/L, 182 mg/L,respectively). Pseudomonas Agar P Medium with 2% glycerol (w/v) was used as control. Also, EPS productions of these Pseudomonas strains have been determined in sugarbeet molasses [2-6% w/v concentrations (2-6 g molasses/100 mL water)I as a complex carbonsource. The highest EPS production of the G1 strain (397 mg/L) was found in 6% w/v molasses concentration according to the control, while the highest EPS production of the G12 strain (367 mg/L) was found in 4% w/v molasses concentration according to thecontrol. The use of sugarbeet molasses as an inexpensive alternative to glycerol was efficient.
机译:在这项研究中,研究了铜绿假单胞菌G1和恶臭假单胞菌G12菌株在含2%甘油的假单胞菌琼脂P培养基上产生胞外多糖(EPS)的情况。检测了这些菌株的EPS产量(分别为192 mg / L,182 mg / L)。含有2%甘油(w / v)的假单胞菌琼脂P培养基用作对照。同样,这些假单胞菌菌株的EPS产量已在甜菜糖蜜中确定[浓度为2-6%w / v(2-6 g糖蜜/ 100 mL水)作为复杂的碳源。根据对照,G1菌株的最高EPS产量(397 mg / L)以6%的糖蜜浓度发现,而G12菌株的最高EPS产量(367 mg / L)以4%w / v糖蜜浓度按对照。使用甜菜糖蜜作为甘油的廉价替代品是有效的。

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