Effects of fluoxetine on mast cell morphology and protease-1 expression in gastric antrum in a rat model of depression.

摘要

AIM: To investigate the effects of fluoxetine on depression-induced changes of mast cell morphology and protease-1 (rMCP-1) expression in rats. METHODS: A Sprague-Dawley rat model of chronic stress-induced depression was established. Fifty experimental rats were randomly divided into the following groups: normal control group, fluoxetine + normal control group, depressed model group, saline + depressed model group, and fluoxetine + depressed model group. Laser scanning confocal microscopy (LSCM) immunofluorecence and RT-PCR techniques were used to investigate rMCP-1 expression in gastric antrum. Mast cell morphology was observed under transmission electron microscopy. ANOVA was used for statistical analysis among groups. RESULTS: Morphologic observation indicated that depression induced mast cell proliferation, activation, and granule hyperplasia. Compared with the normal control group, the average immunofluorescence intensity of gastric antrum rMCP-1 significantly increased in depressed model group (37.4 +/- 7.7 vs 24.5 +/- 5.6, P < 0.01) or saline + depressed model group (39.9 +/- 5.0 vs 24.5 +/- 5.6, P < 0.01), while there was no significant difference between fluoxetine + normal control group (23.1 +/- 3.4) or fluoxetine + depressed model group (26.1 +/- 3.6) and normal control group. The average level of rMCP-1mRNA of gastric antrum significantly increased in depressed model group (0.759 +/- 0.357 vs 0.476 +/- 0.029, P < 0.01) or saline + depressed model group (0.781 +/- 0.451 vs 0.476 +/- 0.029, P < 0.01 ), while no significant difference was found between fluoxetine + normal control group (0.460 +/- 0.027) or fluoxetine + depressed model group (0.488 +/- 0.030) and normal control group. Fluoxetine showed partial inhibitive effects on mast cell ultrastructural alterations and de-regulated rMCP-1 expression in gastric antrum of the depressed rat model. CONCLUSION: Chronic stress can induce mast cell proliferation, activation, and granule hyperplasia in gastric antrum. Fluoxetine counteracts such changes in the depressed rat model.