Enzymatic synthesis of fucose-containing disaccharides employing the partially purified #alpha#-L-fucosidase from Penicillium multicolor

摘要

The #alpha#-L-Fucp-(1->3)-D-Glcp NAc disaccharide structure is a vital core unit of the oligosaccharide components of glycoconjugates isolated from human milk and blood group substances.#alpha#-L-Fucosidase from Penicillium multicolor catalyses the transfer of L-fucose from donor structures such as #alpha#-L-FucpOpNP and #alpha#-L-Fucp Fto various Glcp NAc derivatives and Glcp,forming #alpha#(1->3)linkages.The synthesis of several bologically relevant disaccharides including #alpha#-L-Fucp-(1->3)-#alpha#-D-GlcpNAcOMe,#alpha#-L-Fucp-(1->)#alpha#-D-GlcpNAcOAll,#alpha#-L-Fucp-(1->3) -#beta#-D-Glcp NAcOAll,#alpha#-L-Fucp-(1->3)-D-Glcp has beenachieved in up to 34%yields by application of this enzyme.