Effect of molecular weight and degree of chitosan deacetylation on the preparation and characteristics of chitosan thermosensitive hydrogel as a delivery system

摘要

A thermosensitive hydrogel has been prepared with chitosan and αβ-glycerophosphate (αβ-GP) which could be transited from solution into gel at 37 °C. The thermosensitive characteristics, appearance and structure of the hydrogel were affected by concentration, molecular weight and degree of deacetylation (DD) of chitosan. Chitosan, MW 1360kDa, DD 75.4%, solution concentration 2% was optimal for preparation of chitosan-αβ-glycerophosphate (CS-αβ-GP) thermosensitive hydrogel. The appearance of the hydrogel became more compact and regular with increasing concentration and chitosan MW. Model drugs release from CS-αβ-GP hydrogel prepared by the drug being added into chitosan solution (method I) was slower than that from hydrogel prepared by the drug being directly added into chitosan hydrogel (method II).The release rate for both adriamycin and 6-mercaptopurine from CS-αβ-GP hydrogel decreased with the increase of MW of the chitosan. The hydrophilic model adriamycin was released 60-70% over 24 h which was slower than that of the hydrophobic model 6-mercaptopurine. Therefore it was projected that the CS-αβ-GP hydrogel should be an ideal sustained release system especially for hydrophilic drugs.