The Effects of Various BSA Levels in Different Media on Development in In Vitro Culture of Mouse Embryos

摘要

Various BSA (bovine serum albumin) levels and media effects on development of 2-cell stage mouse embryos in in vitro culture were investigated in this 2 stage study In the first stage, 1400 2-celled mouse embryos were cultured in 2 various BSA-bearingculture media for 96 h and directly observed microscopically. In the second stage, 1024 2-celled mouse embryos cultured for the same period and cell numbers (nuclei of cells) were determined by staining techniques. Embryos were cultured in both stages in M 16 and Whitten's media containing 0 (control), 0.3, 1, 3, 9, 18 and 36 mg/ml of BSA The highest blastocyst rate was 94.57% ± 7.43, observed in 3 mg/ml BSA-containing Whitten's medium group (P < 0.05) The highest hatching + hatched blastocyst rates were obtained in the same group with 67.70% ± 25.22. The highest nucleus number in the M 16 medium was in the 1 mg/ml BSA group (95.45 ± 9.61). In the M16 medium group, the 3 mg/ml BSA group showed the highest development rate in the first stage of thestudy; however, the nucleus numbers in this group (81.37 ± 18.31), were significantly lower than the nucleus numbers of the 1 mg/ml BSA group (P < 0.05). It was concluded that the optimal benefit to in vitro culture of 2-ceil mouse embryos could be maintained when BSA was used as a protein source at 1 mg/ml and 3 mg/ml doses, and that Ivl 16 or Whitten's media could be used successfully as a culture medium Developmental evaluations of in vitro cultured mouse embryos could be confusing when only the native route was employed, and a staining technique could give more reliable results.