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首页> 外文期刊>Transactions of the Royal Society of Tropical Medicine and Hygiene >Combined diagnostic methods identify a remarkable proportion of asymptomatic Leishmania (Leishmania) chagasi carriers who present modulated cytokine profiles.
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Combined diagnostic methods identify a remarkable proportion of asymptomatic Leishmania (Leishmania) chagasi carriers who present modulated cytokine profiles.

机译:组合的诊断方法可鉴定出无症状利什曼原虫(Leishmania)chagasi携带者的比例显着,这些携带者表现出可调节的细胞因子谱。

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摘要

Peripheral blood samples of 138 co-habitants from 25 families with recently diagnosed cases of visceral leishmaniasis in the Metropolitan Region of Belo Horizonte, Minas Gerais, Brazil, were analyzed by indirect fluorescent antibody test (IFAT), rK39 and Leishmania chagasi Enzyme Linked Immunosorbent Assay (ELISA), intradermal skin-test and Polymerase Chain Reaction (PCR) over a 12-month period. The cumulative positivity was significantly higher by PCR (29.7%) than by IFAT, rK39 ELISA, L. chagasi ELISA and intradermal skin-test (5.1%, 6.5%, 14.5% and 2.9%, respectively). In addition, the cytokine profile was measured in 16 of the 138 volunteers, of whom eight were asymptomatic carriers and eight were non-infected co-habitants. The innate immunity cells from asymptomatic carriers displayed, upon in vitro antigenic stimulation, a modulated increase in cytokine synthesis that was distinct from that observed in non-infected volunteers. This study suggests that the identification of a large proportion of asymptomatic carriers is facilitated when more than one diagnostic method is applied and that a mixed pattern of immune response is correlated with clinical status of asymptomatic individuals. These observations suggest also that asymptomatic infection by L. chagasi is a frequent event and that control programs could benefit by including this indicator in their interventions.
机译:通过间接荧光抗体试验(IFAT),rK39和利什曼原虫查加西酶联免疫吸附法分析了来自巴西米纳斯吉拉斯州贝洛奥里藏特大都会地区25个家庭的138名同居居民的外周血样本,这些病例最近被诊断为内脏利什曼病。 (ELISA),皮内皮肤测试和聚合酶链反应(PCR),历时12个月。 PCR的累积阳性率(29.7%)明显高于IFAT,rK39 ELISA,南美锥虫(L.chagasi)ELISA和皮内皮肤试验(分别为5.1%,6.5%,14.5%和2.9%)。此外,在138名志愿者中的16名中测量了细胞因子谱,其中8名是无症状携带者,而8名是未感染的同居者。在体外抗原刺激下,来自无症状携带者的先天免疫细胞显示出细胞因子合成的调节增加,这与未感染志愿者中观察到的明显不同。这项研究表明,当采用一种以上的诊断方法时,有助于鉴定大部分无症状携带者,而且免疫反应的混合模式与无症状个体的临床状况相关。这些观察结果还表明,恰加斯乳杆菌无症状感染是一个经常发生的事件,控制程序可通过将该指标纳入其干预措施而受益。

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