Objective To investigate the Changes and Value of the Proportion of CD4 + CD25 + regulatory T cells (Treg) in HBeAg( + ) and HBeAg(-) chronic asymptomatic hepatitis B virus carriers(ASC). Methods Patients with HBeAg( + ) ASC(n =50) , HBeAg( - ) ASC( n = 50 ) and normal subjects ( n = 20 ) were recruited in this study. Flow cytometric analysis was used to detect the quantification of peripheral CD3+ , CD4+ and CD8+ T lymphocyte subgroup, CD4+ CD25+ Treg. Results The proportion of CD3+ , CD4+ , CD8+ T lymphocyte subgroup has no significant difference among healthy controls, HBeAg( + ) and HBeAg( - ) ASC. The ratio of CD4 and CD8 in HBeAg( + ) patients was elevated, but there was no significant difference between healthy controls and HBeAg( + ) patients. Contrast to normal subjects, the ratio of CD4+ CD25+ Treg increases in HBeAg(+) ASC, but decreases obviously in HBeAg(-) ones(P <0.05). Conclusion Although having normal hepatic function, HBeAg( + ) and HBeAg( - ) ASC have different ratio of peripheral CD4 + CD25 + Treg , Our findings suggest that HBeAg( + ) and HBeAg( - ) ASC stand in different immune state.%目的 探讨HBeAg(+)与HBeAg(-)HBV携带者外周血中CD4+ CD25+调节性T淋巴细胞(Treg)的变化及其意义.方法HBeAg(+)与HBeAg(-)HBV携带者两组各50例患者,应用流式细胞仪测定外周血CD3+T淋巴细胞所占比例、CD4+和CD8+T淋巴细胞所占比例及其比值、CD4+ CD25+ Treg所占比例；以同期门诊健康体检者20例为健康对照组.结果 与健康对照组相比,HBeAg(+)与HBeAg(-)HBV携带者,在外周血CD3+T淋巴细胞、CD4+与CD8+T淋巴细胞所占比例差异无统计学意义.CD4+与CD8+T淋巴细胞比值,在HBeAg(+)HBV携带者有升高趋势,但与HBeAg(-)HBV携带者和对照组比较差异无统计学意义.HBeAg(+)HBV携带者,CD4+ CD25+ Treg所占比例较对照组明显升高,而HBeAg(-)HBV携带者,CD4+ CD25+ Treg所占比例较对照组明显降低,差异均有统计学意义.结论 尽管肝功能在正常范围,HBeAg(+)与HBeAg(-)HBV携带者外周血CD4+ CD25+ Treg比例不同,HBeAg(+)HBV携带者高于健康对照组,而HBeAg(-)HBV携带者低于健康对照组,提示HBeAg(+)与HBeAg(-)HBV携带者处于不同的免疫状态.
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