干扰素治疗慢性乙型肝炎患者HBeAg转阴率和CD4+CD25+调节性T淋巴细胞水平的关系

摘要

Objective To investigate the role of CD4+ CD25 + T regulatory cells in hepatitis B e antigen ( HBeAg) serological conversion during anti - hepatitis B virus (HBV) treatment using interferon. Methods Fifty - six HBeAg - positive chronic HBV patients were administered interferon - alpha 2b (5 000 000 U) intramuscularly every other day for one year. CD4 + CD25 + T regulatory cells were detected by flow cytometry before treatment and 48 weeks after treatment initiation. Results The level of CD4 + CD25 + T regulatory cells was significantly reduced by interferon - alpha 2b treatment in patients with HBeAg conversion (before: 11.99% ±3.25% vs 48 weeks; 9. 12% 2.45% ; P<0.05). However, no decrease in the level of T regulatory cells was observed in the group without HBeAg conversion. Conclusion In chronic HBV, interferon treatment decreases the level of CD4 + CD25 + T regulatory cells in patients with HBeAg seroconversion, but not in patients without HBeAg seroconversion.%目的 观察干扰素抗病毒治疗前后慢性乙型肝炎患者外周血CD4+CD25+调节性T细胞(CD4+CD25+Treg)表达水平的变化和HBeAg转阴的关系.方法 选择用干扰素α-2b治疗的HBeAg阳性慢性乙型肝炎56例,分别于治疗前、48周时检测外周静脉血CD4+CD25+Treg占CD4+T细胞的比例,同时检测HBeAg、ALT和HBVdNA载量.结果 抗病毒治疗48周后HBeAg转阴组CD4+CD25+rreg水平[(9.12±2.45)％]较治疗前[(11.99±3.25)％]明显下降(P＜0.01)；而HBeAg未转阴组较治疗前无明显下降(P＞0.05).结论 慢性乙型肝炎患者经干扰素α-2b抗病毒治疗,HBeAg血清转换者CD4+CD25+Treg水平降低,而未转阴者Treg下降不明显.