Production of Lignocellulytic Enzymes from Spent Mushroom Compostof Pleurotus eryngii

摘要

The lignocellulytic enzymes including a-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), beta-xylosidase (EC 3.2.1.37), beta-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) were extracted fromspent mushroom compost (SMC) of Pleurotus eryngii Different extraction buffers and conditions were tested for optimal recovery of the enzymes. The optimum extraction was shaking incubation (200 rpm) for 2 h at 4°C. alpha-Amylase was extracted with the productivity range from 1.20 to 1.6 Unit/SMC g. Cellulase was recovered with the productivity range from 2.10 to 2.80 U/gf. beta-glucosidase and beta-xylosidase productivities showed lowest recovery producing 0.1 U/g and 0.02 U/g, respectively. The P. eryngii SMCs collected from three different mushroom farms showed different recovery on laccase and xylanse, cellulase. Furthermore, the water extracted SMC was compared to commercial enzymes for its industrial application in decolorization and cellulase activity.