The determination of the accurate immune status of pregnant women is crucial in order to prevent congenital toxoplasmosis. Equivocal results with conventional serological techniques are not uncommon when IgG titers are close to the cut-off value of the test, so that a confirmatory technique is needed. For this purpose, we developed a homemade immunoblot (IB) using soluble extract of Toxoplasma gondii tachyzoites and assessed it by testing 154 positive, 100 negative, and 123 equivocal sera obtained from pregnant women. In order to select the more valuable bands in terms of sensitivity and specificity, we used the Youden Index (YI). The highest Yls were those given by the 32, 36, 98, 21, and 33 bands. The simultaneous presence on the same blot of at least 3 bands showed a much higher YI (0.964) and was adapted as the positivity criterion. The analysis of results showed that our homemade IB correlated well with the commercial LDBIO Toxo II IgG kit recently recommended as a confirmatory test (96.7% of concordance).
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机译：为了预防先天性弓形虫病，确定孕妇的准确免疫状况至关重要。当IgG滴度接近测试的临界值时，使用常规血清学技术获得的结果并不罕见，因此需要一种验证性技术。为此，我们开发了一种使用弓形虫速殖子可溶性提取物的自制免疫印迹（IB），并通过测试从孕妇获得的154阳性，100阴性和123模棱两可的血清对其进行了评估。为了选择在敏感性和特异性方面更有价值的谱带，我们使用了尤登指数（YI）。最高的Yls是32、36、98、21和33频段给出的。在同一印迹上同时存在至少3个条带显示出更高的YI（0.964），并被用作阳性标准。结果分析表明，我们的自制IB与最近推荐作为验证性测试的商业LDBIO Toxo II IgG试剂盒相关性很好（一致性为96.7％）。
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