OPTIMISATION AND VALIDATION OF AN AUTOMATED SOLID PHASE EXTRACTION TECHNIQUE COUPLED ON-LINE TO ENZYME-BASED BIOSENSOR DETECTION FOR THE DETERMINATION OF PHENOLIC COMPOUNDS IN SURFACE WATER SAMPLES

摘要

A fully integrated screening system for phenolic compounds was developed incorporating on-line solid phase extraction, fractionation and biosensor detection. Two different types of biosensors, solid graphite and carbon paste electrodes incorporating the enzyme tyrosinase, were compared and used in the screening system. Interfacing of the solid phase extraction and fractionation with the biosensor detection was given special attention since the biosensors were not compatible with the organic modifier used for desorption of phenols from the solid phase extraction step. The system was validated with conventional analytical techniques. Surface water samples from the Ebro river were spiked with 1, 10, and 25 mu g L(-1) of catechol, phenol, p-cresol, respectively. Three out of seven samples were spiked and the correct samples were identified, containing phenols equivalent to the spiked concentrations. [References: 31]