Ultrafast DMA amplification with a rapid PCR system

摘要

The polymerase chain reaction (PCR) is being used in an ever-increasing number and variety of applications. In parallel with this explosive growth, the technical requirements of the test itself are continuously becoming more and more demanding in terms of speed, efficiency and quality of results. This article describes a new rapid cycle PCR technology that offers substantial advances while still maintaining the traditional advantages of PCR. Practical examples of the powerful performance characteristics of the new system are given. Ever since the invention of the PCR method in 1985 by Kary Mullis and co-workers [1, 2], continuous development has led to the technique becoming a more and more indispensable tool in biological research and routine diagnostics. Significant milestones along this development continuum have been the introduction of heat stable DNA polymerases such as the Taq DNA polymerase [3] and the development of the thermal cycler, which made it possible for the PCR test to be carriedout automatically on numerous samples in parallel.